Protein conformational substates from X-ray crystallography
نویسندگان
چکیده
منابع مشابه
Accessing protein conformational ensembles using room-temperature X-ray crystallography.
Modern protein crystal structures are based nearly exclusively on X-ray data collected at cryogenic temperatures (generally 100 K). The cooling process is thought to introduce little bias in the functional interpretation of structural results, because cryogenic temperatures minimally perturb the overall protein backbone fold. In contrast, here we show that flash cooling biases previously hidden...
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Using the three-dimensional structure of biological macromolecules to infer how they function is one of the most important fields of modern biology. The availability of atomic resolution structures provides a deep and unique understanding of protein function, and helps to unravel the inner workings of the living cell. To date, 86% of the Protein Data Bank (rcsb-PDB) entries are macromolecular s...
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In this article a review on the definition of the X- ray transform and some ofits applications in Nano crystallography is presented. We shall show that the X- raytransform is a special case of the Radon transform on homogeneous spaces when thetopological group E(n)- the Euclidean group - acts on ℝ2 transitively. First someproperties of the Radon transform are investigated then the relationship ...
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Functioning proteins do not remain fixed in a unique structure, but instead they sample a range of conformations facilitated by motions within the protein. Even in the native state, a protein exists as a collection of interconverting conformations driven by thermodynamic fluctuations. Motions on the fast time scale allow a protein to sample conformations in the nearby area of its conformational...
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Conformational substates of proteins are generally considered to play important roles in regulating protein functions, but an understanding of how they influence the structural dynamics and functions of the proteins has been elusive. Here, we investigate the structural dynamics of sperm whale myoglobin associated with the conformational substates using picosecond X-ray solution scattering. By a...
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ژورنال
عنوان ژورنال: Progress in Biophysics and Molecular Biology
سال: 1996
ISSN: 0079-6107
DOI: 10.1016/s0079-6107(97)85629-5